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ActiGraph llc actiwatch 2
Actiwatch 2, supplied by ActiGraph llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2/actiwatch/nct04545606-27-0-2
Average 90 stars, based on 1 article reviews
actiwatch 2 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

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Article Title: Digital intervention for increasing physical activity and reducing sedentary behaviour in cancer survivors: a trial within the Cancer Prevention Study-3 cohort.
Article Snippet: © Author(s) (or their employer(s)) 2025.. No commercial reuse.. See rights and permissions.

Article Title:
Article Snippet: Before the pilot study, we looked into multiple actigraphs (Micro Motionlogger Watch, ActiWatch 2, ActiWatch Spectrum Plus, ActiGraph wGT3X-BT, GENEActiv Original, and MotionWatch 8) and piloted three of those (ActiGraph wGT3X-BT, GENEActiv Original, and MotionWatch 8).

Article Title: Stability and change in latent movement behaviour profiles during adolescence and links with future depressive symptoms.
Article Snippet: Movement behaviours were assessed via Actigraph (AM7164-2.2) accelerometery; depressive symptoms via the Short Mood and Feelings Questionnaire.

Article Title: Intervenção de esportes modificados para melhorar metas de participação e competências de atividade em crianças deambuladoras com paralisia cerebral: um ensaio clínico randomizado.
Article Snippet: Os participantes usaram dispositivos ActiGraph por 7 dias consecutivos (5 dias úteis e 2 dias de final de semana) antes das avaliações.

Article Title: Stability and change in latent movement behaviour profiles during adolescence and links with future depressive symptoms.
Article Snippet: PA was measured using Actigraph AM7164 2.2 accelerometers [Actigraph LLC, Fort Walton Beach, FL, USA] in 2005–2006 when participants were 11.7 years of age (henceforth referred to as age 12; T1) and again when age 13.9 (henceforth 14 years; T2), and age 15.5 (henceforth 16 years; T3).

Article Title: Exploring the relationship between APOEε4 allele and gut microbiota composition and function in healthy adults
Article Snippet: The physical activity levels were assessed as previously described in (Bressa et al. ) using an ActiSleep V.3.4.2 accelerometer (Actigraph, Manufacturing Technology Inc., Shalimar, FL).

Activity Assay:

Article Title: Weight cycling and relative energy deficiency in sport syndrome in an elite female muaythai athlete: a case study
Article Snippet: .. Physical activity and training load data were collected using wearable devices (ActiGraph wGT3X-BT, Garmin HRM-Run 2). ..

Software:

Article Title: Interplay of Physical Activity, Muscle Strength, and Depression in Cognitive Impairment among Korean Older Adults: A Cross-sectional Study
Article Snippet: .. To ensure data accuracy, the 30-Hz data were aggregated into 15-second periods using ActiLife software (ActiGraph R version 6.13.2), and periods of non-wear were identified and removed using the Choi algorithm [ ]. ..



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Thermo Fisher ratiometric calcium indicator dye fura 2 am
Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Inhibition, Staining, Imaging

RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Imaging

Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Activity Assay, Imaging, Enzyme-linked Immunosorbent Assay